Your challenges in chromatography
A distinction is made here between the application areas of analytical, preparative and flash chromatography. All processes require exact dosing of the solvents to produce high-precision results.
The challenge within analytical chromatography
The imprecise switching behaviour of the valves often leads to an imprecise mixing of the mobile phase, which results in an imprecise gradient. The production of valve blocks is complex and therefore cost-intensive due to the machining, mechanical processes. If large numbers of valve blocks are produced, this has a negative effect on the manufacturing costs of the chromatographs.
The challenge for preparative chromatography
The manual process by which the substance is brought from the fraction collector back to the sample injection can often prove to be error-prone. The manual method is much more laborious in terms of personnel and time, as the substances have to be put back into the sample injector in conscientious steps. This slows down the overall process.
The challenge in flash chromatography
Speed and fast results are essential in flash chromatography. The chromatographs used must work economically and deliver a high output within the shortest possible time, as downstream processes depend on them.
Bürkert system solutions ensure precise, repeatable and automated processes
In a high-throughput laboratory, standardised methods for the separation of substances are indispensable. Our solutions comply with standards, are chemically resistant and can be individually adapted to your requirements.
Thanks to precisely working valves, which convince with their service life, high switching accuracy and chemical resistance (FFKM), we realise a reliable and exact gradient mixture and thus the best possible separation results.
Solution low-pressure gradient with type 6650
Our pulse valves allow an automatic bottle change of the solvent. Your advantage: The replacement of the empty vessels can be carried out during operation.
Solution high-pressure gradient with type 6724 pulse
Thanks to individual solutions, we guarantee you a continuous and fast process for the column flow and the distribution of the relevant fractions. The automated return of the substance from the fraction collector back to the separation column also reduces your manual effort. Fast further processing is secured.
Customised solutions for preparative chromatography based on our types:
Our dosing solutions guarantee a high output in a short time and work very economically. The automated cleaning of the distribution blocks accelerates your process. Thanks to fewer interfaces, the risk of leakage is reduced.
The use of Bürkert dosing technologies in chromatography offers you the following advantages:
How Bürkert solutions can shorten your maintenance work and achieve accurate results
With Bürkert solutions, your maintenance costs can be minimised enormously. This is made possible by a 10 times longer service life compared to conventional valves. In addition, the peaks always remain exact over a long period of time and do not show any fluctuations. For you, this means long-term and reliable results.
In a laboratory with an eight-hour working day, the HPLC devices run for approximately six hours. The valves are clocked at 1 Hz. This results in 21,600 switching operations per working day.
Conventional solution and Bürkert solution in comparison
In the conventional solution, one valve reaches 10 million circuits, which means it is ready for use for 463 days. The chromatograph must be calibrated within a year for 8 hours. In contrast, a Bürkert solution achieves 100 million circuits, is ready for use for 4630 days and only needs to be calibrated for one hour a year. This means that you have a savings potential of +90 million circuits, a longer service life of +4167 days and 7 hours less maintenance time so that you can achieve the best results.
Switching behaviour and repeatability
Gradient mixing has a direct influence on the separation of fractions. The peaks reflect the accuracy over the service life at intervals of 6 months.
The gradient mixture of the conventional solution compared to the Bürkert solution
Due to the inaccurate dosing of the gradients, the peaks of the conventional solution blur over time. With the Bürkert solution with type 6650, the gradient mixture remains accurate even after 6 months and thus enables reliable results.
We will find the right solution for your liquid chromatograph. Please contact us to discuss your individual needs.
Your challenges in chromatography